diff --git a/homeassistant/helpers/template.py b/homeassistant/helpers/template.py
index 3e2e3a55c3e..62e9f3fab6a 100644
--- a/homeassistant/helpers/template.py
+++ b/homeassistant/helpers/template.py
@@ -1689,16 +1689,16 @@ def random_every_time(context, values):
 
 def today_at(time_str: str = "") -> datetime:
     """Record fetching now where the time has been replaced with value."""
-    start = dt_util.start_of_local_day(datetime.now())
+    today = dt_util.start_of_local_day()
+    if not time_str:
+        return today
 
-    dttime = start.time() if time_str == "" else dt_util.parse_time(time_str)
+    if (time_today := dt_util.parse_time(time_str)) is None:
+        raise ValueError(
+            f"could not convert {type(time_str).__name__} to datetime: '{time_str}'"
+        )
 
-    if dttime:
-        return datetime.combine(start.date(), dttime, tzinfo=dt_util.DEFAULT_TIME_ZONE)
-
-    raise ValueError(
-        f"could not convert {type(time_str).__name__} to datetime: '{time_str}'"
-    )
+    return datetime.combine(today, time_today, today.tzinfo)
 
 
 def relative_time(value):
diff --git a/tests/helpers/test_template.py b/tests/helpers/test_template.py
index a50884b71c8..bd405acf597 100644
--- a/tests/helpers/test_template.py
+++ b/tests/helpers/test_template.py
@@ -5,6 +5,7 @@ import math
 import random
 from unittest.mock import patch
 
+from freezegun import freeze_time
 import pytest
 import voluptuous as vol
 
@@ -1149,42 +1150,68 @@ def test_utcnow(mock_is_safe, hass):
     assert info.has_time is True
 
 
+@pytest.mark.parametrize(
+    "now, expected, expected_midnight, timezone_str",
+    [
+        # Host clock in UTC
+        (
+            "2021-11-24 03:00:00+00:00",
+            "2021-11-23T10:00:00-08:00",
+            "2021-11-23T00:00:00-08:00",
+            "America/Los_Angeles",
+        ),
+        # Host clock in local time
+        (
+            "2021-11-23 19:00:00-08:00",
+            "2021-11-23T10:00:00-08:00",
+            "2021-11-23T00:00:00-08:00",
+            "America/Los_Angeles",
+        ),
+    ],
+)
 @patch(
     "homeassistant.helpers.template.TemplateEnvironment.is_safe_callable",
     return_value=True,
 )
-def test_today_at(mock_is_safe, hass):
+def test_today_at(mock_is_safe, hass, now, expected, expected_midnight, timezone_str):
     """Test today_at method."""
-    now = dt_util.now()
-    with patch("homeassistant.util.dt.now", return_value=now):
-        now = now.replace(hour=10, minute=0, second=0, microsecond=0)
-        result = template.Template(
-            "{{ today_at('10:00').isoformat() }}",
-            hass,
-        ).async_render()
-        assert result == now.isoformat()
+    freezer = freeze_time(now)
+    freezer.start()
 
-        result = template.Template(
-            "{{ today_at('10:00:00').isoformat() }}",
-            hass,
-        ).async_render()
-        assert result == now.isoformat()
+    original_tz = dt_util.DEFAULT_TIME_ZONE
 
-        result = template.Template(
-            "{{ ('10:00:00' | today_at).isoformat() }}",
-            hass,
-        ).async_render()
-        assert result == now.isoformat()
+    timezone = dt_util.get_time_zone(timezone_str)
+    dt_util.set_default_time_zone(timezone)
 
-        now = now.replace(hour=0)
-        result = template.Template(
-            "{{ today_at().isoformat() }}",
-            hass,
-        ).async_render()
-        assert result == now.isoformat()
+    result = template.Template(
+        "{{ today_at('10:00').isoformat() }}",
+        hass,
+    ).async_render()
+    assert result == expected
 
-        with pytest.raises(TemplateError):
-            template.Template("{{ today_at('bad') }}", hass).async_render()
+    result = template.Template(
+        "{{ today_at('10:00:00').isoformat() }}",
+        hass,
+    ).async_render()
+    assert result == expected
+
+    result = template.Template(
+        "{{ ('10:00:00' | today_at).isoformat() }}",
+        hass,
+    ).async_render()
+    assert result == expected
+
+    result = template.Template(
+        "{{ today_at().isoformat() }}",
+        hass,
+    ).async_render()
+    assert result == expected_midnight
+
+    with pytest.raises(TemplateError):
+        template.Template("{{ today_at('bad') }}", hass).async_render()
+
+    freezer.stop()
+    dt_util.set_default_time_zone(original_tz)
 
 
 @patch(