diff --git a/core/lib/Drupal/Core/Asset/AssetResolver.php b/core/lib/Drupal/Core/Asset/AssetResolver.php
index 7f4b110bc52a..94343886ced2 100644
--- a/core/lib/Drupal/Core/Asset/AssetResolver.php
+++ b/core/lib/Drupal/Core/Asset/AssetResolver.php
@@ -162,16 +162,6 @@ class AssetResolver implements AssetResolverInterface {
     // Sort CSS items, so that they appear in the correct order.
     uasort($css, 'static::sort');
 
-    // Allow themes to remove CSS files by CSS files full path and file name.
-    // @todo Remove in Drupal 9.0.x.
-    if ($stylesheet_remove = $theme_info->getStyleSheetsRemove()) {
-      foreach ($css as $key => $options) {
-        if (isset($stylesheet_remove[$key])) {
-          unset($css[$key]);
-        }
-      }
-    }
-
     if ($optimize) {
       $css = \Drupal::service('asset.css.collection_optimizer')->optimize($css);
     }
diff --git a/core/lib/Drupal/Core/Theme/ActiveTheme.php b/core/lib/Drupal/Core/Theme/ActiveTheme.php
index 9da3e7d14d97..ff1b89e9b970 100644
--- a/core/lib/Drupal/Core/Theme/ActiveTheme.php
+++ b/core/lib/Drupal/Core/Theme/ActiveTheme.php
@@ -62,13 +62,6 @@ class ActiveTheme {
    */
   protected $extension;
 
-  /**
-   * The stylesheets which are set to be removed by the theme.
-   *
-   * @var array
-   */
-  protected $styleSheetsRemove;
-
   /**
    * The libraries provided by the theme.
    *
@@ -109,7 +102,6 @@ class ActiveTheme {
       'engine' => 'twig',
       'owner' => 'twig',
       'logo' => '',
-      'stylesheets_remove' => [],
       'libraries' => [],
       'extension' => 'html.twig',
       'base_theme_extensions' => [],
@@ -123,7 +115,6 @@ class ActiveTheme {
     $this->path = $values['path'];
     $this->engine = $values['engine'];
     $this->owner = $values['owner'];
-    $this->styleSheetsRemove = $values['stylesheets_remove'];
     $this->libraries = $values['libraries'];
     $this->extension = $values['extension'];
     $this->baseThemeExtensions = $values['base_theme_extensions'];
@@ -188,26 +179,6 @@ class ActiveTheme {
     return $this->libraries;
   }
 
-  /**
-   * Returns the removed stylesheets by the theme.
-   *
-   * This method is used as a BC layer to access the contents of the deprecated
-   * stylesheets-remove key in theme info.yml files. It will be removed once it
-   * is no longer needed in Drupal 10.
-   *
-   * @return mixed
-   *   The removed stylesheets.
-   *
-   * @see https://www.drupal.org/node/2497313
-   *
-   * @todo Remove in Drupal 10.0.x.
-   *
-   * @internal
-   */
-  public function getStyleSheetsRemove() {
-    return $this->styleSheetsRemove;
-  }
-
   /**
    * Returns an array of base theme extension objects keyed by name.
    *
diff --git a/core/lib/Drupal/Core/Theme/ThemeInitialization.php b/core/lib/Drupal/Core/Theme/ThemeInitialization.php
index e451d73abff2..ee52887b59d1 100644
--- a/core/lib/Drupal/Core/Theme/ThemeInitialization.php
+++ b/core/lib/Drupal/Core/Theme/ThemeInitialization.php
@@ -179,9 +179,6 @@ class ThemeInitialization implements ThemeInitializationInterface {
       $values['logo'] = $theme->getPath() . '/logo.svg';
     }
 
-    // @todo Remove in Drupal 10.0.x.
-    $values['stylesheets_remove'] = $this->prepareStylesheetsRemove($theme, $base_themes);
-
     // Prepare libraries overrides from this theme and ancestor themes. This
     // allows child themes to easily remove CSS files from base themes and
     // modules.
@@ -306,50 +303,4 @@ class ThemeInitialization implements ThemeInitializationInterface {
     }
   }
 
-  /**
-   * Prepares stylesheets-remove specified in the *.info.yml file.
-   *
-   * This method is used as a BC layer to access the contents of the deprecated
-   * stylesheets-remove key in theme info.yml files. It will be removed once it
-   * is no longer needed in Drupal 10.
-   *
-   * @param \Drupal\Core\Extension\Extension $theme
-   *   The theme extension object.
-   * @param \Drupal\Core\Extension\Extension[] $base_themes
-   *   An array of base themes.
-   *
-   * @return string[]
-   *   The list of stylesheets-remove specified in the *.info.yml file.
-   *
-   * @todo Remove in Drupal 10.0.x.
-   *
-   * @internal
-   */
-  protected function prepareStylesheetsRemove(Extension $theme, $base_themes) {
-    // Prepare stylesheets from this theme as well as all ancestor themes.
-    // We work it this way so that we can have child themes remove CSS files
-    // easily from parent.
-    $stylesheets_remove = [];
-    // Grab stylesheets from base theme.
-    foreach ($base_themes as $base) {
-      if (!empty($base->info['stylesheets-remove'])) {
-        @trigger_error('The theme info key stylesheets-remove implemented by theme ' . $base->getName() . ' is deprecated in drupal:8.0.0 and is removed from drupal:10.0.0. See https://www.drupal.org/node/2497313', E_USER_DEPRECATED);
-        foreach ($base->info['stylesheets-remove'] as $css_file) {
-          $css_file = $this->resolveStyleSheetPlaceholders($css_file);
-          $stylesheets_remove[$css_file] = $css_file;
-        }
-      }
-    }
-
-    // Add stylesheets used by this theme.
-    if (!empty($theme->info['stylesheets-remove'])) {
-      @trigger_error('The theme info key stylesheets-remove implemented by theme ' . $theme->getName() . ' is deprecated in drupal:8.0.0 and is removed from drupal:10.0.0. See https://www.drupal.org/node/2497313', E_USER_DEPRECATED);
-      foreach ($theme->info['stylesheets-remove'] as $css_file) {
-        $css_file = $this->resolveStyleSheetPlaceholders($css_file);
-        $stylesheets_remove[$css_file] = $css_file;
-      }
-    }
-    return $stylesheets_remove;
-  }
-
 }
diff --git a/core/modules/system/tests/modules/theme_test/theme_test.routing.yml b/core/modules/system/tests/modules/theme_test/theme_test.routing.yml
index d08c5ba4d855..1299fe9562e6 100644
--- a/core/modules/system/tests/modules/theme_test/theme_test.routing.yml
+++ b/core/modules/system/tests/modules/theme_test/theme_test.routing.yml
@@ -1,10 +1,3 @@
-theme_test.info_stylesheets:
-  path: '/theme-test/info/stylesheets'
-  defaults:
-    _controller: '\Drupal\theme_test\ThemeTestController::testInfoStylesheets'
-  requirements:
-    _access: 'TRUE'
-
 theme_test.template_test:
   path: '/theme-test/template-test'
   defaults:
diff --git a/core/modules/system/tests/src/Functional/Theme/LegacyStyleSheetsRemoveTest.php b/core/modules/system/tests/src/Functional/Theme/LegacyStyleSheetsRemoveTest.php
deleted file mode 100644
index fde9b59b2326..000000000000
--- a/core/modules/system/tests/src/Functional/Theme/LegacyStyleSheetsRemoveTest.php
+++ /dev/null
@@ -1,45 +0,0 @@
-<?php
-
-namespace Drupal\Tests\system\Functional\Theme;
-
-use Drupal\Tests\BrowserTestBase;
-
-/**
- * Tests the legacy stylesheets-remove key.
- *
- * @group system
- * @group legacy
- */
-class LegacyStyleSheetsRemoveTest extends BrowserTestBase {
-
-  /**
-   * {@inheritdoc}
-   */
-  protected $defaultTheme = 'stark';
-
-  /**
-   * {@inheritdoc}
-   */
-  protected function setUp(): void {
-    parent::setUp();
-    \Drupal::service('theme_installer')->install(['test_legacy_stylesheets_remove']);
-  }
-
-  /**
-   * Tests the stylesheets-remove key.
-   *
-   * @throws \Behat\Mink\Exception\ExpectationException
-   */
-  public function testStyleSheetsRemove() {
-    $this->expectDeprecation('The theme info key stylesheets-remove implemented by theme test_legacy_stylesheets_remove is deprecated in drupal:8.0.0 and is removed from drupal:10.0.0. See https://www.drupal.org/node/2497313');
-    \Drupal::configFactory()->getEditable('system.theme')->set('default', 'classy')->save();
-    $this->drupalGet('<front>');
-    $this->assertSession()->responseContains('css/components/action-links.css?');
-    $this->assertSession()->responseContains('css/components/breadcrumb.css?');
-    \Drupal::configFactory()->getEditable('system.theme')->set('default', 'test_legacy_stylesheets_remove')->save();
-    $this->drupalGet('<front>');
-    $this->assertSession()->responseNotContains('css/components/action-links.css?');
-    $this->assertSession()->responseContains('css/components/breadcrumb.css?');
-  }
-
-}
diff --git a/core/modules/system/tests/src/Functional/Theme/ThemeInfoTest.php b/core/modules/system/tests/src/Functional/Theme/ThemeInfoTest.php
index 93242b6d8eba..4437f0b3c41a 100644
--- a/core/modules/system/tests/src/Functional/Theme/ThemeInfoTest.php
+++ b/core/modules/system/tests/src/Functional/Theme/ThemeInfoTest.php
@@ -56,7 +56,7 @@ class ThemeInfoTest extends BrowserTestBase {
   }
 
   /**
-   * Tests stylesheets-remove.
+   * Tests libraries-override.
    */
   public function testStylesheets() {
     $this->themeInstaller->install(['test_basetheme', 'test_subtheme']);
diff --git a/core/modules/system/tests/themes/test_legacy_stylesheets_remove/test_legacy_stylesheets_remove.info.yml b/core/modules/system/tests/themes/test_legacy_stylesheets_remove/test_legacy_stylesheets_remove.info.yml
deleted file mode 100644
index 6cd835e4d591..000000000000
--- a/core/modules/system/tests/themes/test_legacy_stylesheets_remove/test_legacy_stylesheets_remove.info.yml
+++ /dev/null
@@ -1,9 +0,0 @@
-name: 'Theme Legacy Test Stylesheets Remove'
-type: theme
-description: 'Test theme using legacy stylesheets-remove.'
-version: VERSION
-base theme: classy
-package: Testing
-libraries: { }
-stylesheets-remove:
-  - '@classy/css/components/action-links.css'
diff --git a/core/tests/Drupal/Tests/Core/Theme/RegistryTest.php b/core/tests/Drupal/Tests/Core/Theme/RegistryTest.php
index d18c35677566..3cc86665c4c3 100644
--- a/core/tests/Drupal/Tests/Core/Theme/RegistryTest.php
+++ b/core/tests/Drupal/Tests/Core/Theme/RegistryTest.php
@@ -110,7 +110,6 @@ class RegistryTest extends UnitTestCase {
       'path' => 'core/modules/system/tests/themes/test_theme/test_theme.info.yml',
       'engine' => 'twig',
       'owner' => 'twig',
-      'stylesheets_remove' => [],
       'libraries_override' => [],
       'libraries_extend' => [],
       'libraries' => [],
@@ -123,7 +122,6 @@ class RegistryTest extends UnitTestCase {
       'path' => 'core/tests/fixtures/test_stable/test_stable.info.yml',
       'engine' => 'twig',
       'owner' => 'twig',
-      'stylesheets_remove' => [],
       'libraries_override' => [],
       'libraries_extend' => [],
       'libraries' => [],