diff --git a/tools/toolchains/__init__.py b/tools/toolchains/__init__.py
index 3aa3cc5758..7b69b8230c 100644
--- a/tools/toolchains/__init__.py
+++ b/tools/toolchains/__init__.py
@@ -612,6 +612,7 @@ class mbedToolchain:
                             break
 
             # Add root to include paths
+            root = root.rstrip("/")
             resources.inc_dirs.append(root)
             resources.file_basepath[root] = base_path